|
KMID : 1094720130180030528
|
|
Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 3 p.528 ~ p.532
|
|
|
Expression of bacterial xylose isomerase in Saccharomyces cerevisiae under galactose supplemented condition
|
|
Park Ju-Yong
Park Dong-Jun
Chung Bong-Woo
Min Ji-Ho
|
|
|
Abstract
|
|
|
|
We constructed recombinant Saccharomyces cerevisiae harboring the xylose isomerase (XI) gene isolated from Clostridium phytofermentans to metabolize xylose and use it as a carbon and energy source. In this study, the effect of supplementation using co-substrate such as glucose or galactose on xylose utilization was studied in recombinant S. cerevisiae. Glucose, which is transported with high affinity by the same transport system as is xylose, was not affected by the heterologous expression of XI, thus xylose utilization was not observed in recombinant S. cerevisiae. However, supplemental galactose added to the recombinant S. cerevisiae stimulated xylose utilization as well as the expression of XI protein. Recombinant S. cerevisiae consumed up to 23.48 g/L of xylose when grown in media containing 40 g/L of xylose and supplemented with 20 g/L of galactose. These cells also produced 15.89 g/L of ethanol. Therefore, expression of the bacterial XI in recombinant S. cerevisiae was highly induced by the addition of supplemental galactose as a co-substrate with xylose, and supplemented galactose enabled the yeast strain to grow on xylose and ferment xylose to ethanol.
|
|
|
KEYWORD
|
|
|
xylose isomerase, galactose, xylose utilization, Saccharomyces cerevisiae
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|